Propagation of Human Corneal Endothelial Cells ? A Novel Dual Media Approach.

In this study, we describe a novel dual media culture approach for the in vitro expansion of primary human CECs. Initial characterization included analysis of growth dynamics of CECs grown in either proliferative (M4) or maintenance (M5) medium. Subsequent comparisons were performed on isolated CECs cultured in M4?alone against cells expanded using the dual media approach. Further characterizations were performed using immunocytochemistry, quantitative real?time PCR and gene expression microarray. At the third passage, results showed that human CECs propagated using the dual media approach were homogeneous in appearance, retained their unique polygonal cellular morphology, and expressed higher levels of corneal endothelium?associated markers in comparison to CECs cultured in M4?alone, whic…

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