1,25-Dihydroxyvitamin D decreases HTRA1 promoter activity in the rhesus monkey – a plausible explanation for the influence of vitamin D on age-related macular degeneration?

In this study, we predicted 9 binding sites for the vitamin D-dependent transcription factor Vitamin D Receptor in the rhesus HTRA1 promoter, one of which is destroyed by the rs196357513-risk allele. As patients with vitamin D deficit are at increased risk for age-related macular degeneration, a luciferase assay in transiently transfected ARPE19-cells was performed to evaluate the influence of the SNP rs196357513 and of 1,25-dihydroxyvitamin D on the rhesus monkey HTRA1 promoter activity. This revealed that the luciferase activity of the promoter construct containing the rs196357513 wild type allele was significantly reduced after vitamin D stimulation. An in silico analysis and literature search imply that this regulation could also play a role in human HTRA1 expression. Moreover, HTRA1 p…

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